Lab Protocols

In-fusion homemade

Fly Genomic DNA extraction

 

Collections of Drosophila Protocols

果蝇实验手册

Information center of fly research at Flybase

Protocols from Flyrani.org

Protocols from Bio-protocol

Methods from FlyBook

Drosophila Developmental Biology Methods

Biological Procedures Online

Springer Nature Experiments

Springer Link： Collection of eBooks and Protocols

Bioz (Collections of reagents and protocols such as antibodies and their working concentrations.)

 

 

Collections of Drosophila stocks

Centers of fly stocks: Bloomington Drosophila Stock Center (BDSC) , Kyoto Stock Center (DGRC) , Vienna Drosophila Resource Center (VDRC) , NIG-fly, FlyROF, Tsinghua Fly Center .

 

Reviews of Drosophila Biology

More comprehensive reviews of fly biology can be found at FlyBook, Interactive Fly.

 

Microscopy Related

FPbase: Database of fluorescent proteins (FPs), their sequences and properties.

FBDB: Database of genetically encodable fluorescent protein biosensors.

OptoBase: Database of genetically encodable optogenetic molecules.

MicroscopeU (Here, you can learn almost everything about the Microscopes.

 

 

Basic Fly Biology

 

 

Basic Fly Techniques

 

Excellent Online Courses

 

 

Fly Bioinformatics

Review of fly bioinformatic tools: Data pushing: a fly-centric guide to bioinformatics tools.

Collections of fly bioinformatic tools at DRSC Center.

Online tool for fly genome engineering: CRISPR fly design.

 

 

Immunohistochemistry

 

 

Western Blot

Find antibodies for fly proteins: Developmental Studies Hybridoma Bank (DSHB).

 

 

RT-PCR

Find qPCR primers for fly genes: FlyPrimerBank.

 

 

ELISA

 

 

Cloning

Collection of plasmids and cloning techniques can be found at Addgene and DGRC (fly vectors at Drosophila Genomics Resource Center).

 

Tissue Culture

More videos of tissue culture can be found at JoVE, Abnova, and DGRC (Collection of fly cell lines and protocols)

 

 

Seahorse XF

 

 

Cryostat

 

 

Biacore SPR

 

 

Thunder Microscope

 

 

Luxendo MuVi SPIM

 

 

Image Processing and Analysis

More online resources of software applications important for our study: Fiji/ImageJ (Macro), Fiji Scripting Tutorial , R / Rstudio , MatLab (need a USTC license) / Octave, and Python.

 

We strongly suggest interested people to find more protocols of Drosophila handling, dissection, and staining at JoVE and iBiology Techniques.